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dc.contributor.advisorSalazar Salvatierra, María Elena
dc.contributor.authorPérez Alarcón, María Elizabeth
dc.date.accessioned2013-11-20T20:15:37Z
dc.date.available2013-11-20T20:15:37Z
dc.date.issued2013
dc.identifier.urihttps://hdl.handle.net/20.500.12672/3388
dc.description.abstractSe analizaron 60 muestras de Salchicha tipo Huacho de los Mercados de Abastos del Cercado de Lima, con la finalidad de determinar la incidencia de Listeria monocytogenes. Estas muestras se llevaron al Laboratorio de Microbiología de la Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, en recipientes estériles, refrigerados y se procesaron dentro de las 24 horas de colectadas. Para el análisis microbiológico se utilizó la técnica según USDA- FSIS, que consta de tres fases: Pre-enriquecimiento, Enriquecimiento de la muestra y siembra en agares selectivos, pruebas Bioquímicas, Catalasa, Gram positivos y la prueba del CAMP para su confirmación. La incidencia de L. monocytogenes en Salchicha tipo Huacho, en los Mercados de Abastos del Cercado de Lima es de 78%, siendo un valor considerable y que supera a los valores reportados en estudios a nivel internacional. Estos resultados permiten considerar que la Salchicha tipo Huacho se constituye como un alimento de riesgo potencial para la Salud Pública.
dc.description.abstract--- 60 samples of the Huacho Sausage from the Food Markets in Cercado de Lima, in Lima, Peru, were analyzed in order to determine the incidence of Listeria monocytogenes. These samples were taken to the Laboratory of Microbiology to the Faculty of Pharmacy and Biochemistry at the National University of San Marcos. They were taken in sterile containers for being refrigerated. Then they were processed within 24 hours of being collected. The USDA-FSIS technique was used for microbiological analysis, which consists of three phases: Pre-enrichment of the sample, Sample Enrichment and Reseeding on selective agars. At the same time, some biochemical tests were applied, such as the positive Catalase test, the test of Great Positive, and CAMP test to confirm the results. The incidence of the Listeria monocytogenes in the Huacho Sausage, from the Food Markets in Cercado de Lima, is 78%. This is quite high, as it exceeds the values reported in international studies. These results support the conclusion that the Huacho Sausage is a food with a potential risk to the public health.
dc.description.uriTesis
dc.language.isospa
dc.publisherUniversidad Nacional Mayor de San Marcos
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceRepositorio de Tesis - UNMSM
dc.sourceUniversidad Nacional Mayor de San Marcos
dc.subjectListeria Monocytogenes
dc.subjectSalchicha tipo Huacho
dc.subjectAgar Oxford
dc.titlePrevalencia de Listeria Monocytogenes en salchichas tipo Huacho provenientes de los mercados de abastos del Cercado de Lima
dc.typeinfo:eu-repo/semantics/masterThesis
thesis.degree.nameMagíster en Ciencias de los Alimentos
thesis.degree.grantorUniversidad Nacional Mayor de San Marcos. Facultad de Farmacia y Bioquímica. Unidad de Posgrado
thesis.degree.disciplineCiencias de los Alimentos
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#3.01.05
dc.publisher.countryPE
renati.advisor.dni08675623
renati.advisor.orcidhttps://orcid.org/0000-0002-5661-4752
renati.levelhttps://purl.org/pe-repo/renati/level#maestro
renati.typehttps://purl.org/pe-repo/renati/type#tesis


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